The Activation Marker CD137 (4-1BB) Identifies a Highly Active Subset of Donor Lymphocytes Against Acute Myeloid Leukemia. Haitham Abdelhakim, MD.
cells expressing the activation marker CD137 (4-1BB) after exposure to overlapping PRAME peptides as a rapid method of ex vivo expansion for clinical use (Figure 1). CD137 is a costimulatory molecule and a member of the tumor necrosis factor receptor (TNFR) family. Transient increased expression is seen on cells that have been
CD137 ligand (CD137L) is expressed by antigen presenting cells (APC) as a transmembrane protein and transmits activating signals into APC. In this study we investigated the effects of CD137L signaling in microglia, the resident APC in the central nervous system. In 2019-11-08 · CD137 was originally discovered in 1989 and reported as a cell surface protein mainly located on activated T cells . Interaction of CD137 with its ligand (CD137L, also known as TNFSF9 or 4-1BBL) on activated antigen-presenting cells (APCs) could lead to bidirectional activation that promotes immunity against cancer . Immunotherapy of cancer with immunomodulatory agents is achieving significant efficacy in an important fraction of patients. The stimulatory inducible receptor of T and NK lymphocytes known as CD137 or 4-1BB is being stimulated with agonist antibodies to enhance antitumor immunity in clinical trials.
CD137 (teal surface) comprises 4x cysteine-rich domains (CRD), a transmembrane domain (TM) and a cytoplasmic domain (CD). The CD137 signaling complex requires organization of the receptor into trimers by CD137L (orange surface). A multimeric 2012-07-16 · CD137 (4-1BB, TNFRSF9), a member of the tumor necrosis factor (TNF) receptor family, is a potent T cell co-stimulatory molecule. CD137 ligand (CD137L) is expressed by antigen presenting cells (APC) as a transmembrane protein and transmits activating signals into APC. In this study we investigated the effects of CD137L signaling in microglia, the resident APC in the central nervous system.
HIV replication is also under cellular regulation, and activation of the T cell by a mitogen or antigen also activates the virus . CXCL16 and CD137 in Atherosclerosis.
CD137 Can Act as a Surrogate Marker for T Cell Activation. CD137 (TNFSFR9) was originally identified as a molecule induced on the surface of activated mouse and human CD4+ and CD8+ T cells, with its expression undetectable on non-activated T cells. It is also found on both NK and dendritic cells.
guide RNA. Interestingly, activation of CD137-CD137L was negatively correlated with CyPA expression in vivo and in vitro. Stimulating CD137-CD137L interaction significantly increased CyPA, which was concurrent with the upregulation of proinflammatory cytokines, chemokines and matrix metalloproteinases and resulted in the promotion of atherosclerosis in ApoE-/- mice. CD137 is a costimulatory molecule transiently expressed on activated T cells after mitogen or antigen stimulation that can be exploited for isolating antigen-specific T cells as reported in mouse models.
In this issue of Clinical Cancer Research, Ye and colleagues use CD137 as a marker to successfully enrich and expand tumor-specific T cells from cancer tissues for adaptive immunotherapy (1). Adoptive cell therapy (ACT) has been very effective in the clinical treatment of advanced melanoma.
However, CD137 is also expressed in a number of other immune cells like B cells, dendritic cells, and monocytes, which hampers the isolation of antigen-specific T cells based solely on this marker. Moreover, the presence of CD137+ 5 regulatory T cells could inhibit an effective expansion of 2013-12-19 · Antibodies which activate the T-cell co-stimulatory receptor CD137 or block the co-inhibitory receptor PD-1 have demonstrated broad anti-tumor effects but have not improved the survival of mice with poorly immunogenic tumors, including the ID8 murine ovarian cancer, when used as single agents [25,39,40]. Se hela listan på academic.oup.com Dynabeads Human T-Activator are coupled with anti-CD3, anti-CD28, and anti-CD137 antibodies.
Transient increased expression is seen on cells that have been
After 16 h, activated cells expressing CD137 were isolated with immunomagnetic beads and cocultured with irradiated CD137 neg fraction in medium supplemented with interleukin (IL)-2, IL-7 and IL-15. Cultured T cells were restimulated with antigen-pulsed autologous cells after 10 days. Pan markers CD3 CD4 CD5 CD7 CD25h CD27 CD28 CD44h CD62L (naïve: high, effector: low, memory: high) CD69h CD127 (IL7Rα) CD134 (OX40)h CD137 (4-1BB)h CD152 (CTLA-4)h CD154 (CD40L) h CD272 (BTLA)h CD278 (ICOS)h CD279 (PD-1)h Mouse CD4 T cell markers 7 h = Upregulated i = Downregulated = Key marker = Subset
Antigen Independent Activation Marker Expression, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations.
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CXCL16 and CD137 in atherosclerosis / Dick Wågsäter. - Örebro Detection of these glycoproteins is a useful marker of infec- tion. HIV replication is also under cellular regulation, and activation of the T cell by a mitogen or antigen also activates the virus .
Activation of the CD137 Pathway in T cells by a CD137 x 5T4 bispecific ADAPTIR™ Molecule Requires Co-engagement of CD137 and 5T4. A) CD137 (NF-kB/luciferase) reporter cells were stimulated with serial dilutions of ALG.APV-527 in the presence of 5T4 or empty vector transfected CHO-K1 cells for 5 hr. ALG.APV-527 induces CD137
ex vivo stimulation using the activation marker CD137 (4-1BB).
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Detection of these glycoproteins is a useful marker of infec- tion. HIV replication is also under cellular regulation, and activation of the T cell by a mitogen or antigen also activates the virus . CXCL16 and CD137 in Atherosclerosis. 6.
Activation of CD137 signaling can stimulate both cytotoxic T cell and NK cell activity. HER2 x CD137 and EphA2 x CD137 DART molecules bind their respective target antigens. Co-culturing of a CD137/ NF-kB reporter cell line with tumor lines expressing HER2 or EphA2 revealed tumor antigen-dependent CD137 pathway activation by HER2 x CD137 and EphA2 x CD137 DART molecules, respectively.